Rapid Determination of plankton biomass Wan deep AlgaeC algae counts and auxiliary Identification System
Phytoplankton is a general term for several species of algae that are suspended in water. As an important member of the aquatic ecosystem, phytoplankton is an important component of fish natural bait. Because phytoplankton is very sensitive to environmental changes, it is also important in environmental monitoring. The algae composition is different in different types of water bodies or different seasons of the same water body. The relative amounts of various algae are constantly changing, and this change has a certain trend. The composition and stock of phytoplankton in water is an important scientific basis for the rational release of farmed fish, which can serve the research and utilization of aquatic ecology.
The current amount of phytoplankton refers to the amount of phytoplankton present in a unit of water at a certain instant. There are two ways of expressing it: the density is expressed in units of numbers (usually in units of L/L ), and the existing amount expressed in mass units of mg/L is biomass. In the past surveys, only the type or quantity of phytoplankton was usually paid attention to, but the biomass was not paid enough attention. Because different water bodies and different types of algae vary greatly in individuals, it is difficult to evaluate the abundance of bait organisms in different water bodies only by the quantity. Therefore, it is more scientific to quantify the biomass of phytoplankton.
There are two types of classical research methods for phytoplankton biomass. One type is biomass "state" measurement (measuring dry weight, cell number and population volume), which theoretically uses the entire phytoplankton as an indicator of biomass, which is less biased and less reliable. The other type is the phytoplankton biomass "group" measurement (measuring phytoplankton cell components). It includes three major components of phytoplankton cells, particulate organic carbon (POC) , particulate organic nitrogen (PON) , determination of particulate organophosphorus and determination of other components of cells such as chlorophyll a , ATP , proteins and other pigments. measuring. This method measures the active components of living cells and accurately reflects the biomass of the population, but it is difficult to reflect the contribution of different phytoplankton species to matter and energy transfer in the ecosystem.
Some foreign scholars have determined the carbon content, cell volume and cell surface area of ​​different phytoplankton cells, and found that the correlation between cell volume and cell carbon content is stronger than that of cell surface area, and established phytoplankton cell volume and cell carbon. The regression equation for the content. Thus, various phytoplankton cell count results are converted into biomass by biomass measurement such as cell volume and carbon content, so that the biomass of phytoplankton community can be reasonably estimated at the species level. The biomass estimation method is widely used: it can understand the structure of phytoplankton biomass and the contribution of different phytoplankton functional groups or species to biomass, which is of great significance for understanding the structure of ecosystems. It can also understand the ecological processes related to phytoplankton community and biomass from the species level, so it is of great significance to understand the function of ecosystems.
Microscopic counting is the most direct method for measuring phytoplankton biomass and is the only method to date to identify and count phytoplankton to species levels. The counting results can be used to define phytoplankton community, analyze population distribution and species composition, and block distribution of time and space in the community. At the same time, the counting result can also convert the number of phytoplankton cells into biomass or energy, but the traditional direct Counting is slow, laborious, and requires considerable taxonomic expertise. To this end, Hangzhou Wan deep Detection Technology Co., Ltd. integrate various finishing the massive domestic public resources, the introduction of superior AlgaeC plankton counts and secondary identification system. The classification system can count the number of plankton, and is equipped with powerful plankton intelligent search library to help personnel quickly and easily statistical classification and identification of plankton, the system also includes efficient phytoplankton biomass measurement modules.
Generally, phytoplankton individuals are extremely small and should not be directly weighed, and their relative cell density is mostly close to 1 , so the cell volume can be calculated by the geometric volume formula with similar morphology, namely: cell volume conversion method or geometric volume fitting method. Literature [1] shows that this method is more reliable and feasible for the estimation of phytoplankton cell volume. Currently Wan deep AlgaeC plankton counts and secondary identification system using this method already built 32 different geometric model, and common algae code corresponding to a multi-model, based on the generic name automatically recommend the geometric model chosen, the The whole process of biomass determination is simple and convenient (see the annex for details of the measurement steps). This calculation method is also similar to the biomass estimation for zooplankton.
references
[1] Sun Jun . Model of marine phytoplankton cell volume and surface area and its converted biomass [D]. Ocean University of China, 2004
[2] Zhao Wen . Aquatic Biology . Beijing: China Agricultural Press, 2005
annexBiomass measurement step
1. After using the 10,000-deep AlgaeC system to identify the type and establish the meter, select the item to be measured, right-click the pop-up menu and click on the measurement volume , as shown below:
2 , open the volume measurement form, the system gives the recommended model according to the type, or you can choose from the built-in 32 geometric models according to actual needs .
3. According to the model diagram, the parameters can be measured to obtain the volume. The length of the line, the length of the curve, and the drag anchor point can be measured to adjust the measured value. For the difficult to measure species, the three-dimensional scale ratio provided by the original reference is converted.
4. After the measurement is completed, click the OK button and the measurement volume will appear in the count. After complete statistics on the number of horizons Department, Wan deep AlgaeC system to generate inspection reports. A sample screenshot is as follows:
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