Introduction to the immunoaffinity column of Aokin, Germany

AOKIN Mycotoxin Immunoaffinity Column

one. Introduction

Mycotoxins are toxic metabolites produced by molds that have strong carcinogenicity and reduce immunity. Common mycotoxins are mainly aflatoxins, ochratoxin, vomiting hormone, trichothecenes (T-2 toxin), zearalenone, beadedomycin, fumonisin and the like. Food and feed are very susceptible to contamination by mycotoxins, and this problem has increasingly become a major problem that cannot be ignored in modern agricultural production.

two. Detection method

The main methods for the determination of mycotoxins are thin layer chromatography, high performance liquid chromatography, fluorometry and enzyme-linked immunosorbent assays. The experimental principle and operation process of these methods are different, and the factors that affect the accuracy and sensitivity of the experimental results are also diverse. One of the common and most important factors influencing this is the purity of the sample. The high-purity sample has a low impurity content, which reduces the interference factor during the experiment, thereby improving the reliability and stability of the experimental results. Therefore, the selection of appropriate extraction and purification methods is essential for mycotoxin detection.

Traditional methods for purifying mycotoxins use organic solvent extraction and chemical adsorption methods. These methods have many shortcomings:

1. The specificity is not high, the purification efficiency is low, and the purification effect is poor.
2. Experimental sensitivity and repeatability are poor.
3. The operation process is cumbersome, complicated, long-term, and labor intensive.
4. The use of organic solvents is likely to cause environmental pollution and cause greater damage to operators.

The latest national standard method "immune affinity column method" better solves the above deficiencies. This method uses a specific immune reaction as a separation means, which greatly improves the purity and purification efficiency of the sample, and ensures the reliability and stability of the test results. . This method has good authority and versatility, and has been listed as a standard method by various testing organizations at home and abroad. This technology has the following characteristics:

1. Using monoclonal immunization technology, the mycotoxin can be separated specifically, the separation efficiency and recovery rate are high, and the correctness and reliability are strong.
2. The experiment has good stability and strong repeatability.
3. The purification process is simple and fast, and can process a large number of samples in a short time.
4. The experimental equipment is light, with high degree of automation, simple operation, low requirements for the experimenter, and can be used in small test sites.
5. The process is safe and reliable without toxic solvents.
6. Wide range of applications to handle a wide range of complex samples.

three. Aokin mycotoxin immunoaffinity column

Germany aokin company has developed a series of mycotoxin immunoaffinity column products with its strong scientific research strength and advanced production technology. This series of products has passed many scientific experiments and has been certified by many foreign official testing institutions. Such as the American Association of Public Analytical Chemists (AOAC), the United States Department of Agriculture Federal Grain Testing Center (FGIS).

The aokin immunoaffinity column can selectively absorb mycotoxins from the sample solution to efficiently purify the mycotoxins present in the sample; the sample solution purified by the column can be directly used for chromatography or ELISA. Detection. It brings convenience to any kind of detection method and greatly improves the accuracy and stability of the detection method. The aokin immunoaffinity column series products include the following features:

1. Select highly specific and high affinity monoclonal antibodies to ensure high purity samples
2. The affinity column has a large capacity and a high antibody content, which increases the amount of sample adsorption and improves the purification efficiency.
3. Allow large volume samples to be purified by column, which has excellent concentration effect on low concentration samples, thus effectively improving the detection limit of each method.
4. The product has passed a large number of scientific experiments and has good stability and reliability. The sample recovery rate reaches 90%-95%.
5. The product is versatile and suitable for a variety of buffer systems without the need for complex and toxic reagents.
6. Simple and fast operation, a single sample takes only 10-15 minutes. It is highly adaptable and does not require a specific experimental environment.
7. Purified samples are suitable for ELISA, high performance liquid chromatography, fluorometry, etc.

The aokin mycotoxin immunoaffinity column product list is as follows :

The specific performance parameters and related standard numbers are shown in the following table:

four. Aokin product customer base and service

The aokin immunoaffinity column method is an internationally advanced method for pretreatment and purification of samples, and has been widely used in many authoritative testing institutions abroad. With excellent performance and good price advantage, aokin products have been gradually accepted by domestic inspection and quarantine departments and large and medium-sized enterprises. They have been widely used in inspection and quarantine bureaus and many food and feed enterprises, and have been recognized by customers. .

Dog Shampoo

Puppy Shampoo,Flea Shampoo,Flea Shampoo For Dogs,Homemade Dog Shampoo

Jiangxi Welton Pet Products Co., Ltd. , https://www.weltonpetcare.com