With the rapid development of edible mushroom production, many producers are also learning how to produce strains while they are engaged in cultivation of fungi. This method of self-combination, cost reduction, and income increase is worth advocating. However, at present, the strain production technology is still in the hands of a small number of people. Beginners often take a lot of detours because of poor technology and equipment. The author's four problems that should be noted in the production of strains are briefly described below for reference.
First, the introduction of mother-breeding "four wants". First, introductions must be made to regular, trustworthy research institutes and strain manufacturers, so that the quality of strains can be guaranteed. The second is to clarify the characteristics of bacteria species, to grasp the appropriate temperature range of mushrooming, to be aware of, timely feeding materials sowing. Third, after the introduction of strains, we must make a mushroom test to test the adaptability of strains to ensure that scale production is foolproof. Fourth, after some varieties are preserved in the refrigerator, they need to be rejuvenated and then expanded to the original species. Otherwise, it will be difficult for the species to survive. According to experiments, Coprinus comatus, Agaricus blazei, Stropharia, Flammulina velutipes, fungus and other species are all safer after rejuvenation and expansion of the original species.
Second, culture ingredients formula should be scientific and reasonable. Whether it is the production of original species or cultivated species, the proportion of raw materials must be accurate, and the carbon-nitrogen ratio of the culture materials must be reasonable. In particular, the ratio of nitrogen must not be exceeded. Otherwise, the free ammonia in the bottle and bag cannot be eliminated after bottling and bagging, and the hyphae will not be able to colonize and grow, resulting in the failure of strain production.
Third, sterilization should be thorough, inoculation should be sterile. The bacterial culture medium is sterilized under normal pressure and should be kept at a temperature of 100°C for 8 to 10 hours. The autoclave pressure should be maintained for more than 1.5 hours after reaching 1.5 kg/cm2. Inoculation should be performed strictly according to aseptic procedures.
Fourth, the germination phase should be controlled temperature. The production of strains in high temperature seasons, high natural temperatures, and cultivation of bacteria under conventional conditions, control of elevated temperature is a crucial part. The first is that the inoculation should be conducted at a low temperature in the morning and in the evening. Second, when the strains are placed, there should be a certain gap between the bottle and the bottle or between the bag and the bag, which is conducive to heat dissipation. If the bottle and bag are in close contact, the respiratory heat of the mycelium cannot be effectively distributed, which will inevitably lead to the occurrence of high-temperature burning bacteria, which will hinder the growth and development of the mycelia, and will be sparse and inefficient. The strain quality will be severely impaired. Third, the training room should be selected air convection, well-ventilated room, when the temperature is high, ventilation can be cool at any time. Fourth, in the hot season, bottle and bag should not be overlaid on the top, and it must not be stacked too high. Otherwise, it is extremely unfavorable for heat dissipation.
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